Protein Concentration Lab Report Example | Topics and Well Written Essays - 1000 words

Protein Concentration - Lab floor ExampleThe two most common examples are -helix and -sheet (fig. 1)Proteins are not completely rigid systems and they crack between several related structures while they perform their biological functions or roles. Such changes are often induced by the binding of a substrate to the proteins active site.The Biuret reagent is composed of potassium hydroxide (KOH), squealer (II) sulphate (CuSO4) and potassium atomic number 11 tartrate (KNaC4H4O64H2O). This reagent is used in a protein assay, a colorimetric test to fixate protein preoccupancy by detecting the Cu2+ ion with the help of spectrometric methods. In the presence of proteins, this blue reagent turns purple, while when thither are short-chain polypeptides in solution it turns pink MadSci Network.A spectrometer is an optical instrument used to verse properties of light (intensity, polarisation, etc) over a specific range of the electromagnetic spectrum (in this experiment UV-Vis at a 560nm ). The self-sufficing variable is generally the wavelength of the light expressed as nanometers. These instruments are used in spectroscopy to flier the interaction between radiation and matter.In this experiment, UV/Vis spectroscopy is used to determine the concentration of a solution of proteins with the aforementioned Biuret agent. The Beer-Lambert law (A=lc, where A is the absorbance, is the absorption coefficient, l is the distance light travels through the material, c is the concentration of the species that absorbs the light) establishes that the absorbance of any solution is directly proportional to the concentration of the solution Sheffield Hallam University. To establish the concentration of a solution, it is necessary to know first how the absorbance changes with the concentration. In order to achieve this, the calibration persuade must be determined. AIMSProtein solutions are colourless so ceasenot be easily determined by simple colorimetric methods. Biuret reagent (copper sulphate, sodium hydroxide, and sodium potassium tartrate) reacts with compounds containing two or more peptide bonds to give a purple-violet colour, so this reaction can be used for a colorimetric method for estimating the concentration of protein in solution.This experiment involves treating a series of proteins of known concentration with Biuret reagent. The data obtained may be used to draw a standard curve. This curve can then be used to estimate the concentration of 2 protein solutions for which the concentrations are not known.METHODSYou break been provided with(a)protein standard solution (albumin) 10 mg/ml(b) albumin solutions X and Y of unknown concentration(c) Biuret reagent(d) de-ionised waterPrepare the following in labelled test tubes, mix well and leave for 20-30 legal proceeding at room temperatureTubeABCDEFGProtein standard (ml)43210--De-ionised water (ml)01234--Unknown albumin X (ml)-----4-Unknown albumin Y (ml)------4Biuret reagent (ml)6666666Read the a bsorbance at 540nm for solutions A - G (first using tube E as the blank to zero the